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	Provedor de dados ArchiMer (28) Autor Kaas, Raymond (28) Baron, Regis (8) Cadoret, Jean-paul (8) Bougaran, Gael (6) Le Dean, Loic (6) Lukomska, Ewa (6) Berard, Jean-baptiste (5) Picot, Laurent (5) Arhaliass, Abdellah (4) Thiery, Valerie (4) Vauchel, Peggy (4) Arbault, Suzanne (3) Le Roux, Karine (3) Legrand, Jack (3) Pasquet, Virginie (3) Picquet, Marie-lise (3) Robert, Rene (3) Serive, Benoit (3) Arhaliass, A. (2) Barbaroux, Olivier (2) Mais... Palavra-chave Alginate (4) Laminaria digitata (4) Microalgae (4) Pigments (3) Acidic enzymatic proteolysis (2) Algue (2) Alkaline extraction (2) Apoptosis (2) Bio-refinery (2) Cancer (2) Extraction (2) Intrinsic viscosity (2) Isochrysis (2) MAE (2) Microwave (2) Shrimp cuticles (2) Undaria pinnatifida (2) Alga (1) Alkaline extraction kinetics (1) Allophycocyanin (1) Mais... Tipo do documento Text (28) Ano 2020 (1) 2019 (1) 2017 (2) 2015 (2) 2013 (1) 2012 (3) 2011 (2) 2010 (2) 2009 (1) 2008 (2) 2007 (1) 2004 (1) 2003 (1) 2001 (2) 1992 (1) 1990 (2) 1980 (3) Mais... País France (28) Idioma Inglês (17) Francês (11)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 28 Primeira ... 1 2 ... Última Digital expression profiling of novel diatom transcripts provides insight into their biological functions ArchiMer Maheswari, Uma; Jabbari, Kamel; Petit, Jean-louis; Porcel, Betina M.; Allen, Andrew E.; Cadoret, Jean-paul; De Martino, Alessandra; Heijde, Marc; Kaas, Raymond; La Roche, Julie; Lopez, P.; Martin-jezequel, Veronique; Meichenin, Agnes; Mock, Thomas; Parker, Micaela Schnitzler; Vardi, Assaf; Armbrust, E. Virginia; Weissenbach, Jean; Katinka, Michael; Bowler, Chris. Background: Diatoms represent the predominant group of eukaryotic phytoplankton in the oceans and are responsible for around 20% of global photosynthesis. Two whole genome sequences are now available. Notwithstanding, our knowledge of diatom biology remains limited because only around half of their genes can be ascribed a function based onhomology-based methods. High throughput tools are needed, therefore, to associate functions with diatom-specific genes. Results: We have performed a systematic analysis of 130,000 ESTs derived from Phaeodactylum tricornutum cells grown in 16 different conditions. These include different sources of nitrogen, different concentrations of carbon dioxide, silicate and iron, and abiotic stresses such as low temperature and low... Tipo: Text Ano: 2010 URL: http://archimer.ifremer.fr/doc/00017/12810/9758.pdf Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups ArchiMer Serive, Benoit; Nicolau, Elodie; Berard, Jean-baptiste; Kaas, Raymond; Pasquet, Virginie; Picot, Laurent; Cadoret, Jean-paul. Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37... Tipo: Text Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48416/48627.pdf Optimisation de l'enrichissement nutritif pour la culture d' Isochrysis galbana affinis Tahiti ArchiMer Bougaran, Gael; Le Dean, Loic; Loizel, Céline; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud. La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. La solution d'enrichissement de Conway (SEC), élaborée en 1966 par Walne, est largement utilisée pour la production de T -iso en batch. Cependant, les apports nutritifs de cette solution d'enrichissement ne sont pas spécifiquement optimisés pour la culture de cette algue, participant à engendrer des coûts de production élevés (Valenzuala-Espinoza et al., 1999 ; Fabregas et al., 1986) et des effluents inépuisés. Dans les 15 dernières années, plusieurs auteurs sont parvenus à réduire le coût de solutions... Tipo: Text Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41519/40727.pdf Possibilité de culture de l'algue alimentaire Undaria Pinnatifida sur les côtes de France - L'ensemencement ArchiMer Perez, Rene; Kaas, Raymond. En 1971, un ostréiculteur de l'étang de Thau découvrait dans son parc à huîtres une algue qui lui paraissait étrange pour le lieu : il s' agissait en effet de l'espèce Undaria pinnatifida (MARVEY) SURINGAR (fig. 1), originaire du Japon, sans doute introduite involontairement sous forme de plantules ou de spores en même temps que le naissain d'huîtres importé d'Extrême-Orient. Tipo: Text Ano: 1980 URL: http://archimer.ifremer.fr/doc/00046/15738/13140.pdf Elements for optimizing a one-step enzymatic bio-refinery process of shrimp cuticles: Focus on enzymatic proteolysis screening ArchiMer Baron, Regis; Socol, Marius; Kaas, Raymond; Arhaliass, A.; Rodriguez Del Pino, J.; Le Roux, Karine; Donnay-moreno, Claire; Berge, Jean-pascal. This article complements an earlier work published in 2015 Baron et al. (2015) that showed the interest of a shrimp shells bio-refining process. We compare here the effect of eleven commercial proteases at pH 3.5 or 4.0 on a residual amount of shrimp shells proteins after 6 hours at 50 °C. The two pH are obtained when respectively 40 and 25 mmol of formic acid are added to 5 g of mild dried shell. Deproteinisation yield above 95% are obtained. Residual amino acids profile in the solid phase was identical for the eleven proteases except for pepsin which was similar to the raw material profile. A significant relative increase in the proportion of Glycine is observed for the ten other cases. Likewise, shapes of size exclusion chromatograms of the dissolved... Tipo: Text Palavras-chave: Bio-refinery; Shrimp cuticles; Acidic enzymatic proteolysis. Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49957/50530.pdf Optimisation de la production en écloserie de mollusques : Mise au point d'un pilote industriel de production phytoplanctonique en continu et des techniques associées pour leur intégration en écloserie commerciale ArchiMer Angeri, S.; Bougaran, Gael; Connan, Jean-paul; Jouin, Jean-claude; Kaas, Raymond; Le Dean, Loic; Legrand, J.; Loubiere, K.; Lukomska, Ewa; Olivo, Erell; Picquet, Marie-lise; Pruvost, J.; Robert, Rene. Les travaux présentés dans le cadre de ce rapport d'activité rendent compte des avancées réalisées sur le plan (1) biologique, avec d'une part une étude sur le comportement du genre Chaetoceros en culture continue et, d'autre part, les résultats d'expérimentations sur les performances d'élevages larvaires réalisés sur la base de cultures de microalgues en continu, et (2) technologique, avec la continuité des actions de développement du photobioréacteur destiné aux écloseries commerciales ainsi que des périphériques indispensables à son intégration dans le secteur aquacole. Tipo: Text Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41518/40726.pdf How haptophytes microalgae mitigate vitamin B-12 limitation ArchiMer Nef, Charlotte; Jung, Sebastien; Mairet, Francis; Kaas, Raymond; Grizeau, Dominique; Garnier, Matthieu. Vitamin B-12 (cobalamin) can control phytoplankton development and community composition, with around half of microalgal species requiring this vitamin for growth. B-12 dependency is determined by the absence of cobalamin-independent methionine synthase and is unrelated across lineages. Despite their important role in carbon and sulphur biogeochemistry, little is known about haptophytes utilization of vitamin B-12 and their ability to cope with its limitation. Here we report the first evaluation of B-12 auxotrophy among this lineage based on molecular data of 19 species from 9 families. We assume that all species encode only a B-12-dependent methionine synthase, suggesting ubiquitous B-12 auxotrophy in this phylum. We further address the effect of... Tipo: Text Ano: 2019 URL: https://archimer.ifremer.fr/doc/00504/61523/65398.pdf Technique de culture pour les cotes bretonnes de l'algue alimentaire Undaria pinnatifida. Tableau de marché - étude économique ArchiMer Perez, René; Kaas, Raymond; Barbaroux, Olivier; Arbault, Suzanne; Le Bayon, Nicolas; Moigne, J. Introduction Starting in 1986, the ANVAR contributed well appreciated financial support in order to set up an original seeding technique characterized by artificial seeding production in a laboratory. This advantageous new way of seeding, i.e. independency from nature, yield increase and true competitiveness compared to traditional methods, nevertheless requires strict adherence to a procedure progressively elaborated during numerous trials in laboratory as well as at sea. The program ended in July 1989 after testing the cultivation protocol on a 2 hectare exploitation scale, in order to better detect what could be improved to greatly increase productivity, facilitate the work of the harvester and to better adapt the operating mode to environmental... Tipo: Text Palavras-chave: Economic survey; Brittany; Farming technique; Seaweed; Undaria pinnatifida; Etude économique; Bretagne; Technique de culture; Algue; Undaria pinnatifida. Ano: 1990 URL: http://archimer.ifremer.fr/doc/1990/rapport-1659.pdf Study on the microalgal pigments extraction process: Performance of microwave assisted extraction ArchiMer Pasquet, Virginie; Cherouvrier, Jean-rene; Farhat, Firas; Thiery, Valerie; Piot, Jean-marie; Berard, Jean-baptiste; Kaas, Raymond; Serive, Benoit; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent. The performance of microwaves irradiation (MAE and VMAE) to extract pigments from two marine microalgae was compared to conventional processes (cold and hot soaking and ultrasound-assisted extraction). Pigments were quantified by RP-HPLC and extraction performance was assessed regarding rapidity, reproducibility and extraction yields. Scanning electron microscopy was used at all extraction steps to assess the impact of the process on microalgal cell integrity. Freeze-drying and pigments extraction preserved microalgae cell integrity (except sonication) and evoked agglutination in superposed cells layers. All processes performed on Dunaliella tertiolecta (chlorophyte) lead to rapid pigments extraction, and equivalent pigments extraction yields, the absence... Tipo: Text Palavras-chave: Pigments; Microalgae; Microwave; Extraction; Chlorophyll; Fucoxanthin; Carotene; MAE; VMAE. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00029/14059/11533.pdf Culture en continu de Isochrysis galbana affinis Tahiti ArchiMer Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Picquet, Marie-lise; Kaas, Raymond; Muller-feuga, Arnaud. La prymnésiophycée Isochrysis galbana affinis Tahiti (T -iso) est une micro algue marine classiquement utilisée comme nourriture de larves de mollusques et de proies vivantes de poissons d'aquaculture dans la plupart des écloseries produisant des juvéniles de ces espèces. Aujourd'hui, de nombreuses écloseries de mollusques et de poissons l'utilisent en association avec d'autres espèces telles que Pavlova lutheri, Chaetoceros calcitrans et Skeletonema costatum. Isolée à Tahiti par JL Martin en 1975 (comm. pers.), cette algue s'est rapidement imposée dans la nutrition larvaire des crevettes et des mollusques en raison de sa croissance rapide en conditions tropicales et de ses qualités nutritionnelles, en relation avec sa forte teneur en acides gras... Tipo: Text Ano: 2001 URL: http://archimer.ifremer.fr/doc/00304/41508/40716.pdf Emergence d'une filière de culture de macro-algues en Bretagne et problème relatif à une espèce non indigène, le wakame (Undaria pinnatifida) ArchiMer Antoine, Loic; Lemoine, Maud; Boulben, Sylviane; Kaas, Raymond; Laurans, Martial; Viard, Frédérique; Potin, Philippe. La synthèse de l’expertise collégiale menée par l’Ifremer et la station biologique de Roscoff permet de préconiser le maintien des cultures de Undaria pinnatifida dans les zones déjà mises en culture à condition de se cantonner aux pratiques culturales des exploitants installés depuis longtemps, avec un suivi et sans expérimenter de nouvelles pratiques pour lesquelles il n’y a pas de recul. Là où des essais ont eu lieu en 2010 et 2011 (Projet Chaco), il serait nécessaire de faire rapidement un état des lieux de la présence ou l'absence d'Undaria pinnatifida en milieu naturel afin de mesurer l'impact de ces essais. En l’état actuel des connaissances il n’y a pas de données scientifiques qui permettent de garantir le respect de l’article 24 du R(CE) N°... Tipo: Text Ano: 2012 URL: http://archimer.ifremer.fr/doc/00086/19740/17380.pdf Dynamical modelling of a reactive extrusion process: Focus on residence time distribution in a fully intermeshing co-rotating twin-screw extruder and application to an alginate extraction process ArchiMer Baron, Regis; Vauchel, Peggy; Kaas, Raymond; Arhaliass, Abdellah; Legrand, Jack. The context of this study is the modelling of reactive extrusion process based on an alginate extraction protocol. Residence Time Distribution (RTD) is one important part to predict the kinetics of reactive compounds. A simple model is proposed to predict RTD in fully intermeshing co-rotating twin-screw extruders without reaction. This model, which can be easily extended to reactive case in a future work, is based on the extension of an axial dispersion model, including control parameters (screw speed and flow rate) and geometrical parameters (screw profile and die design). Simulations were performed for various operating and geometrical conditions so as to illustrate possibilities offered by the proposed model. Validation was conducted for two different... Tipo: Text Palavras-chave: Alginate; Extraction; Extrusion; Mathematical modelling; Residence time distribution; Simulation; Laminaria digitata. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11592/8985.pdf Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells ArchiMer Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent. The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of... Tipo: Text Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin. Ano: 2013 URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf La culture des algues marines dans le monde ArchiMer Perez, René; Kaas, Raymond; Campello, Francois; Arbault, Suzanne; Barbaroux, Olivier. Shouldn't Earth, the human planet, be called the algae planet? First appearing 3.5 billion years ago, algae have always been objective allies of life and humans. Brought about by algae, oxygen and the ozone layer allowed plant and animal life to develop in oceans and on continents. They offer spawning grounds and feeding grounds for marine fauna.... Tipo: Text Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/rapport-4402.pdf Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts ArchiMer Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent. Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at... Tipo: Text Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis. Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti ArchiMer Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Kaas, Raymond; Baron, Regis. Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and... Tipo: Text Palavras-chave: Isochrysis; Cell quota; Photosynthesis; Steady state; Continuous culture. Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-597.pdf Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea ArchiMer Nef, Charlotte; Henry, Céline; Nicolau, Elodie; Bérard, Jean-baptiste; Hervé, Fabienne; Caruana, Amandine; Kaas, Raymond; Mairet, Francis; Garnier, Matthieu. Cobalamin (vitamin B12) is a cobalt-containing enzymatic cofactor involved in methionine synthesis. Provided only by select bacteria and archaea in marine systems, this vitamin is known to limit primary production in different oceanic areas. Understanding the consequences of cobalamin limitation on phytoplankton physiology is of great interest, notably for cobalamin-dependent haptophytes that significantly contribute to oceanic carbon fixation and sulfur cycle through dimethyl sulfonio propionate (DMSP) synthesis. Here, the effect of cobalamin limitation was compared to nitrogen limitation on the model haptophyte Tisochrysis lutea grown in chemostats, combining comparative proteomics with the analysis of major macromolecules and specific osmolytes. Our... Tipo: Text Palavras-chave: Cobalamin; Vitamin B-12; Haptophyte microalgae; Tisochrysis lutea; DMSP; Chemostat; Comparative proteomics; Metabolism. Ano: 2020 URL: https://archimer.ifremer.fr/doc/00654/76575/77711.pdf Les consequences de l'echouement de l'Amoco Cadiz" sur les peuplements algaux exploitables ArchiMer Kaas, Raymond. Introduction. The amount of hydrocarbons dumped into the sea is currently estimated at 6 to 16 million tons per year. This pollution comes from various sources: Chronic discharges associated with human activity such as oil tanker deballasting or discharges from coastal petroleum processing facilities; natural chronic discharges; accidental discharges like leaks coming from drilling well heads or oil tanker accidents. Natural spills happen, in general, in a relatively unobtrusive way and their effects are quickly diminished by significant dilution and rapid degradation of the various components. Oil spills, however, are characterised by a massive and occasional supply of hydrocarbons. The "Amoco Cadiz" shipwreck fits in with this last case and has seemed... Tipo: Text Palavras-chave: Seaweeds; Pollution Effects; Marine Pollution; Oil Spill. Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/publication-1950.pdf Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries ArchiMer Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul. Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal... Tipo: Text Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles ArchiMer Baron, Regis; Socol, Marius; Arhaliass, A.; Bruzac, Sandrine; Le Roux, Karine; Del Pino, J. Rodriguez; Berge, Jean-pascal; Kaas, Raymond. We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99 % of demineralization yield... Tipo: Text Palavras-chave: Bio-refinery; Acidic enzymatic proteolysis; Shrimp cuticles; Demineralization kinetics and modeling. Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39244/37809.pdf Registros recuperados: 28 Primeira ... 1 2 ... Última

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